The one end (5’) of the probe contains a fluorophore while the other end (3’) contains the quencher molecule which is in close proximity of the fluorophore. The chemistry of the TaqMan probe is based on the exonuclease activity of the Taq DNA polymerase enzyme. ![]() ![]() The TaqMan probe is one of the most popular types of probe chemistry available nowadays.Ī type of hydrolysis probe, commonly used in the real-time PCR assay, and developed by Kary Mullis, himself- the founder of PCR. TaqMan probe, scorpion probe and molecular beacons are commonly used DNA probes in the realtime PCR. The computational software and high-end microscopes used to detect the results. The DNA probes are adopted for detection of the microorganism present in a sample and DNA quantification.ĭNA probes are the first choice in the FISH- fluorescence in situ hybridization in which probes are allowed to hybridize directly on the chromosome. The DNA probe can also be synthesised chemically or prepared using the amplification method or using the cDNA library. 100 to 10000bp long complementary DNA sequence is used to detect the presence of complementary DNA sequence.įor that, the single-stranded probe is first prepared by denaturing it and employed it for hybridization on the nitrocellulose paper. ![]() The DNA probe is used to detect the presence of the DNA or a gene present in a sample. Based on our sample, the nucleic acid hybridization probe might be a DNA probe or RNA probe: DNA probe:
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